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KMID : 1129720220390040142
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Korean Journal of Acupuncture
2022 Volume.39 No. 4 p.142 ~ p.151
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Proteomic Analysis for Neuroprotective Effect of Gastrodia elata Blume in the Substantia Nigra of Mice
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Bae Chang-Hwan
Kim Hee-Young
Lee Han-Ul
Seo Ji-Eun
Yoon Dong-Hak
Kim Seung-Tae
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Abstract
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Objectives : Parkinson’s disease (PD) is a neurodegenerative disorder threatening the quality of life and highly occurred in over 65 years old. Gastrodia elata Blume (GEB), a traditional medicine used for the treatment of headache and convulsion, has been reported to have neuroprotective effect. This study was designed to investigate the neuroprotective effect of GEB and the proteomic changes in the substantia nigra (SN) of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice.
Methods : Male eleven-week-old C57BL/6 mice were intraperitoneally injected with 30 mg/kg of MPTP at 24-h intervals for 5 days. Two hours after the daily MPTP injection, the mice were orally administered 800 mg/kg of GEB extract, which continued for 7 days beyond the MPTP injections, for a total of 12 consecutive days. Two hours after the final GEB administration, the brain samples were collected, and dopaminergic neuronal death and proteomic changes in the SN were evaluated.
Results : GEB prevented the MPTP-induced dopaminergic neuronal death and regulated the expression of 11 proteins including thimet oligopeptidase, T-complex protein 1, glycine tRNA ligase, and pyruvate kinase isozymes M1.
Conclusions : GEB prevents MPTP-induced dopaminergic neuronal death by regulating the proteins in the SN.
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KEYWORD
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Parkinson’s disease, Gastrodia elata Blume, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, two-dimensional electrophoresis, substantia nigra
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